following evoglow-kits are available:
evoglow basic kit
The basic kit was composed for easy cloning of the evoglow-genes into the customer-specific host- and vector systems. It comprises three different variants of evoglow-proteins, either with and without a STOP-codon (6 plasmids) on high-copy plasmids. The plasmids replicate well in E. coli and are ideal templates for PCR. They furthermore bear a variety of restriction sites for direct cloning in specific vector-systems.
evoglow express kit
The evoglow express kit comprises a variety of plasmids designed for efficient expression in a wide host range of gram-negative host organisms. The proteins can be expressed either under control of the inducible T7-promotor as well as in a constiutive manner (kan-promotor).
evoglow fusion kit
The fusion kit was designed to create transcriptional protein fusions. It contains plasmids on which the evoglow-gene are flanked by symmetrical multiple cloning sites (MCS) which enables cloning via a singular restriction site i.e. for conducting promotor activity studies or further physiological investigations.
evoglow yeast kit (NEW!!)
The evoglow Yeast kit was created to enable expression of the fluorescent proteins in Yeasts and related organisms. It comprises plasmids on which the evoglow genes are controlled by different yeast promotors (Gal and Act) and you can choose between plasmids that contain a single evoglow gene or a fusion of two genes per plasmid.
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